The Department of Neurology is seeking a Research Technologist. The Research Technologist will be working in a molecular virology (HIV-1, biosafety level 2) laboratory and will utilize a solid foundation in basic laboratory techniques and experience with molecular biology and primary cell culture which requires a high level of rigor and reproducibility. The projects require care in data collection, preparation of reports and interfacing with sponsors. . .

Specific Duties & Responsibilities

• Mouse breeding and husbandry.
• Handle and perform blood draw and injections on mice infected with viral pathogens.
• Isolation of peripheral blood lymphocytes and monocytes from human blood.
• Perform in vitro infection studies with HIV-1.
• Perform general molecular biology techniques such as cloning, qPCR, and Western blotting.
• Perform specialized techniques including multicomponent single-cell labeling, confocal and high-content microscopy, and multi-color flow cytometry.
• Will provide training to other individuals who may be assigned to the laboratory on a regular or temporary basis

Additional Knowledge, Skills & Abilities

• Works effectively as a team-member and exhibits excellent interpersonal, organizational, and self-management skills
  

• Capable of exercising independent judgment while participating in the planning of experiments and interpreting results.
  

• Isolation of PBMCs and culture of primary human cells; sterile technique.
• Must be willing to work under Biohazard Level 2 Safety conditions with HIV-1.
• Must display willingness to learn and implement new technologies.
• Occasional irregular work hours consistent with research needs related to the in vivo studies with mice is required.
• Ability for self-management, integrity and professionalism.

• Bachelor's Degree in Biology, Chemistry or related field.

• Some related post-undergraduate work experience preferred.
• Experience and or interest in conducting single-cell bioinformatics is desirable.
  

• Experience and or interest in in vivo studies with mice.

Classified Title: Research Technologist
Role/Level/Range: ACRO40/E/02/CC
Starting Salary Range: Min $15.50 - Max $26.00 HRLY ($43,160 targeted; Commensurate with experience)
Employee group: Full Time
Schedule: M-F 8:30 am - 5:00 pm
Exempt Status:Non-Exempt
Location:School of Medicine Campus
Department name: SOM Neuro Neuroimmunology
Personnel area: School of Medicine

Please refer to the job description above to see which forms of equivalency are permitted for this position. If permitted, equivalencies will follow these guidelines:
JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.

**Applicants who do not meet the posted requirements but are completing their final academic semester/quarter will be considered eligible for employment and may be asked to provide additional information confirming their academic completion date.

The successful candidate(s) for this position will be subject to a pre-employment background check. Johns Hopkins is committed to hiring individuals with a justice-involved background, consistent with applicable policies and current practice. A prior criminal history does not automatically preclude candidates from employment at Johns Hopkins University. In accordance with applicable law, the university will review, on an individual basis, the date of a candidate's conviction, the nature of the conviction and how the conviction relates to an essential job-related qualification or function.

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The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.

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